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Journal of Clinical Endocrinology & Metabolism, Vol 40, 863-867, Copyright © 1975 by Endocrine Society


ARTICLES

A nonchromatographic radioimmunoassay for 17alpha-hydroxyprogesterone

M Hami, A Rosler and D Rabinowitz

A radioimmunoassay is described for the measurement of 17alpha- hydroxyprogesterone in human plasma. We have employed the principle of "immunologic purification." 17-OHP and related steroids are bound to an excess of antiserum. Steroids with a low affinity for the antibody are extracted by ether. The 17-OHP is subsequently freed from antibody by acid hydrolysis and this extract is assayed by radioimmunoassay.





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Copyright © 1975 by The Endocrine Society