Journal of Clinical Endocrinology & Metabolism, Vol 40, 970-976, Copyright © 1975 by Endocrine Society
Evaluation of azoestriol antisera for estriol measurements in pregnancy plasma directly and after extraction
JR Soares, SJ Gross and RA Bashore
2-(4'-carboxyphenylazo)-estriol antisera were employed to quantitate
pregnancy plasma estriol in ether extracts by single phase radioimmune
assay without chromatography. Utilizing antiserum which crossreacted
minimally even with the monoglucosiduronate metabolites, unextracted plasma
estriol measurements were identical statistically to ether extract
determinations.