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The Journal of Clinical Endocrinology & Metabolism Vol. 86, No. 5 2051-2055
Copyright © 2001 by The Endocrine Society

Original Studies

Loss of Estrogen Receptor ß Expression in Malignant Human Prostate Cells in Primary Cultures and in Prostate Cancer Tissues1

Daniela Pasquali, Valentina Rossi, Dario Esposito, Ciro Abbondanza, Giovanni A. Puca, Antonio Bellastella and Antonio A. Sinisi

Istituto di Endocrinologia (D.P., V.R., D.E., A.B., A.A.S.) and Istituto di Patologia Generale ed Oncologia (C.A., G.A.P.), Seconda Università di Napoli, 80131 Naples, Italy

Address all correspondence and requests for reprints to: Antonio A. Sinisi, M.D., Istituto di Endocrinologia, Seconda Università di Napoli, Building 16, Via Pansini 5, 80131 Naples, Italy. E-mail: antonio.sinisi{at}unina2.it

The aim of this study was to investigate the expression of estrogen receptor (ER) ß and {alpha} genes in normal (N) and malignant (C) primary cultures of human prostate epithelial cells (PEC) and fibroblasts (PFC) and in the prostate tissue donors. Both ERß and ER{alpha} messenger ribonucleic acids were found by RT-PCR analysis in six NPECs and normal prostate tissues and in only one of six CPECs and in the respective cancer tissue donor. The other five CPECs and related cancer tissue donors and all normal and cancer PFCs expressed ER{alpha} messenger ribonucleic acid alone. Immunoblot analysis, using a polyclonal anti-ERß (C-terminal) antibody, demonstrated ERß protein in all NPEC lysates and in one of the six CPECs. ER{alpha} protein was expressed in both NPECs and CPECs when a polyclonal antibody directed against the ER{alpha} N-terminal domain was used. In contrast, ER{alpha} protein was not detected in two of the six CPEC lysates when ER{alpha} C-terminal monoclonal antibodies were used. Using a set of primers designed to amplify the region from exons 6–8, RT-PCR analysis demonstrated the absence of the expected transcript in these cells. The present study shows that the ERß gene is expressed together with ER{alpha} in normal prostates and NPECs, whereas it is barely detectable in prostate cancer and CPECs. Moreover, in some CPECs, the ER{alpha} gene may be transcribed in a changed protein, resulting from the expression of a deletion variant. Together, these data suggest that prostate malignancy is associated with a potential disorder of ER-mediated pathways.




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